Filogenia de Coronavírus canino (CCoV) de cães brasileiros com base em sequências parciais da proteína de membrana. Download Citation on ResearchGate | Canine coronavirus (CCoV) in dogs vaccinated and unvaccinated domiciliated in Pelotas, RS, Brazil Coronavirus canino. Filogenia de Coronavírus canino (CCoV) de cães brasileiros com base em sequências parciais Keywords: coronavirus, dogs, phylogeny, membrane, Brazil.
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Phylogeny of canine coronavirus CCoV from brazilian dogs based on membrane protein partial sequences.
Enteric canine coronavirosis, caused by canine coronavirus CCoVis an endemic infectious disease with worldwide distribution and high morbidity in puppies, with Types I and II CCoV described so far Pratelli et al. CCoV is a member of the genus Alphacoronaviru s, family Coronaviridae, Order Nidovirales, with a single-stranded, positive-sense linear non-segmented RNA with coronaviruus and a helical nucleocapsid formed by the nucleocapsid N protein Masters, The viral envelope contains the coronavirud Mspike S and coronsvirus E proteins, resulting in a virion of about nm in diameter Holmes and Lai, ; Masters, Recently, a pantropic variant of CCoV was described which replicates in the lungs, kidneys, liver, spleen and lymph nodes leading to epidemics and high mortality in puppies Buonavoglia et al.
Although described as a worldwide distributed virus, there are no reports on molecular coronavirs of Brazilian strains of CCoV. This article reports the preliminary results of a survey on the occurrence of CCoV in Brazilian young dogs and the distribution of Types I and II of the virus based on partial sequencing of the membrane protein gene.
Twenty-two fecal samples were collected from dogs with ages ranging from 1 to 6 months, all of them non-vaccinated against CCoV. Sampling was by convenience, without statistical canijo, and the presence or absence of diarrhea was not taken into account at that moment.
[Identification of canine coronavirus (CVC) in Spain]. [Spanish] 
Exclusive rooms were used for sample preparation and RNA extraction, reverse transcription and amplification reactions as well as for electrophoresis in order to avoid amplicon carry-over. Sequences were assembled with Cap-Contig in Bioedit v. Ten strains of the present study were segregated in the Type II cluster, as well as the vaccine strain was included as a positive control, with a bootstrap value of 74, while the remaining 12 strains were segregated in the Type I CCoV cluster with a bootstrap value of 98 Fig.
Regarding the Type II cluster, it can be noticed in Fig. Mean amino acids identities between Type II sub-lineages 01 and 02 were No mixed CCoV Types I and II and no geographic or chronological clustering in found amongst the 22 studied strains, what might mean that the CCoV detected lineages are continually perpetuated in Brazilian dogs. Although Types I and II co-infection were not detected in the present study, this must be interpreted with caution as such corohavirus could only be confirmed or discarded by the use of type-specific conventional or real-time PCRs Pratelli et al.
CCoV M protein is highly immunogenic in the amino terminal Escutenaire et al. As a conclusion, this study demonstrates that both Types I and II CCoV cpronavirus in Brazil, and a supposed Brazilian sub-lineage in Type II will be investigated in further detail by sequencing of other parts of the genome.
The authors are grateful to all dog owners and veterinarians who allowed the collection of the samples. Canine coronavirus highly pathogenic for dogs.
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The molecular biology of coronaviruses.
Identification of canine coronavirus strains from feces by S gene nested PCR and molecular characterization of a new Australian isolate. Two genotypes of canine coronavirus simultaneously detected in the fecal samples of dogs with diarrhea. Development of a nested PCR assay for the detection of canine coronavirus. Canine coronavirus infection in Turkish dog population. Public Healthv. Recebido em 4 de fevereiro de Aceito em 1 de agosto de All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.
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